Composite

Part:BBa_K1194003:Design

Designed by: Rohan Bendre, Aman Kumar, Namit Holay, Mitan Sutradhar   Group: iGEM13_IIT_Madras   (2013-09-23)


pLuxR --> I3A gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
    Illegal NotI site found at 1213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 269
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 11
    Illegal PstI site found at 3027
    Illegal AgeI site found at 327
    Illegal AgeI site found at 690
    Illegal AgeI site found at 1787
    Illegal AgeI site found at 2301
    Illegal AgeI site found at 2571
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to make our part flexible by providing for easy separation of the polycistronic construct into the two component enzymes. For this purpose, we included the restriction enzyme site for Hinf1 in the linker sequence connecting the two genes.


Source

Tryptophan transaminase is derived from Aarabidopsis thaliana. Indole pyruvate decarboxylase is derived from Azospirillum brasilense.


References